Wako和光純藥 BAMBANKER無血清細(xì)胞凍存液 :
操作流程
1)收集對數(shù)生長期的細(xì)胞(5×105-1×107個細(xì)胞)
2)用1mL該細(xì)胞凍存液懸浮細(xì)胞,置于凍存管中,不需預(yù)冷,直接-80℃冷凍保存,也可用液氮快速冷凍細(xì)胞
3)用恒溫箱或者水浴鍋快速復(fù)蘇細(xì)胞
*冷凍細(xì)胞必須處于對數(shù)生長期 Wako和光純藥 BAMBANKER無血清細(xì)胞凍存液 應(yīng)用
【低溫凍存實驗證明以下細(xì)胞保存完好】
● P3U1(mouse myeloma cell line,小樹骨髓瘤細(xì)胞系)、K562(human leukemia cell line,人白血病細(xì)胞
系)、human gastric epithelialcells(人胃上皮細(xì)胞)、human γδT cells(人γδT細(xì)胞);
● Daudi(human B cell line,人B細(xì)胞系)、PC12(rat-derived adrenal pheochromocytoma,大鼠源腎上
腺嗜鉻細(xì)胞瘤)、human B cell line(人B細(xì)胞系);
● OKT4(mouse hybridoma,小鼠雜交瘤細(xì)胞)、monkey B cell line(猴B細(xì)胞系)、Activated lymphocyte
derived from human peripheral blood(人外周血活化淋巴細(xì)胞)、Activated lymphocyte derived from
mouse spleen(小鼠脾臟活化淋巴細(xì)胞)